ALP activity and osteaocalcin content were significantly increased in CCC+BMP7 BMSCs.
CCC+BMP7 BMSCs showed higher proliferation rate. Cell proliferation, alkaline phosphatase (ALP) activity, osteogenesis related gene expression, osteocalcin levels, and calcified nodules were quantified and compared between four groups: untreated controls, BMSCs cultured with CCC complex medium, BMP7-overexpressing BMSCs, and BMP7-overexpressing BMSCs cultured with CCC complex medium (CCC+BMP7). BMP7-overexpressing BMSCs were generated by lentiviral-mediated gene transduction. In the present study, we investigated the combined effect of Colla Comus Cervi (CCC) and BMP7-overexpressing bone marrow-derived mesenchymal stem cells (BMSCs) on osteogenic induction and the treatment of avascular necrosis of the femoral head (ANFH). Wang, Ping Shi, Bin Gao, Zhi-Hui Sun, Tie-Feng Yang, Wu-Bin Han, Shu-Fang Liu, Peng Wang, Lei-Lei Zhao, Bo-Nian Wang, Dan-Dan © 2013 Society of Cosmetic Scientists and the Société Française de Cosmétologie.ĮFFECT OF COLLA CORNUS CERVI COMBINED WITH LV-MEDIATED BMP7 TRANSFECTED BMSCs ON ANFH IN RATS. Therefore, our results showed that combined use of HA and CCC can be helpful for the reconstruction of SE with good stem cell activity and epidermal differentiation. To avoid these limitations, SEs can be used for testing the safety or the efficacy of cosmetic ingredients. Legislation in the EU prohibits marketing cosmetics and personal care products that contain constituents that have been examined through animal experiments. In conclusion, simultaneous presence of HA and CCC contributed to the stem cell activity and epidermal differentiation in the reconstruction of SE. It showed that epidermal differentiation was significantly improved by addition of CCC. Furthermore, filaggrin expression was much increased when CCC was added.
These finding showed that addition of HA and/or CCC may affect the stem cell activity in the reconstruction of skin. Increased staining of integrin Î☖ and Î☡ was variably observed when HA and/or CCC was added to make dermal substitute. But, immunohistochemical staining showed that p63 was significantly increased by addition of HA and/or CCC. Results showed similar morphologic findings when hyaluronic acid (HA) and/or CCC was added. Four different models were constructed according to different composition of dermal substitute.
#CORNUS MAS DEER SKIN#
The aim of this study was to investigate the effects of Cervi cornus Colla (CCC) on the stem cell activity and epidermal differentiation in the reconstruction of skin equivalent. In the reconstruction of skin equivalents (SEs), keratinocyte differentiation is important because epidermal differentiation is closely related with barrier function.
Common name refers to the cherry-like fruits which resemble in color the semi-precious gemstone carnelian (or cornelian).Cervi cornus Colla (deer antler glue) induce epidermal differentiation in the reconstruction of skin equivalents.Ĭhoi, H-R Nam, K-M Kim, D-S Huh, C-H Na, J-I Park, K-C This name was applied to this plant because it was seen as the opposite of Cornus sanguinea, known as the female or wild cornel. The specific epithet mas means "masculine" or "male". Cornus is also the Latin name for cornelian cherry. Genus name comes from the Latin word cornu meaning horn in probable reference to the strength and density of the wood. Fruits may be used for making syrups and preserves. Fruits are edible, although sour tasting fresh off the plant. Fruits are ellipsoid, fleshy, one-seeded berries (drupes to 5/8" long) which mature to cherry red in mid-summer. Ovate to elliptic dark green leaves (to 4" long) typically develop insignificant fall color. Each umbel is surrounded at the base by small, yellowish, petaloid bracts which are much less showy than the large decorative bracts found on some other species of dogwood such as Cornus florida (flowering dogwood) and Cornus kousa (kousa dogwood). Yellow flowers on short stalks bloom in early spring before the leaves emerge in dense, showy, rounded clusters (umbels to 3/4" wide). Scaly, exfoliating bark develops on mature trunks. It typically grows over time to 15-25' tall with a spread to 12-20' wide. Cornus mas, commonly known as cornelian cherry, is a deciduous shrub or small tree that is native to central and southern Europe into western Asia.
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